HtrA1 Antibody (N-term)

• Catalog Number: orb1936763
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-147 amino acids from the N-terminal region of human HtrA1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q92743
• MW: 51287 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB: 1:1000, IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB02326
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine protease HTRA1, 3421-, High-temperature req
• Note: For research use only
• NCBI: NP_002766.1

Anti-HtrA1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-HtrA1 Antibody (N-term) at 1:2000 dilution + mouse brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of HtrA1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

HtrA1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HtrA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.