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Catalog Number | orb1537727 |
---|---|
Category | Antibodies |
Description | HTR3A/5-HT3A Receptor Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%); Mouse (88%); Hamster (81%). |
Concentration | 1 mg/ml |
Dilution range | IHC, IHC-P (5 µg/ml), WB (1 µg/ml) |
Conjugation | Unconjugated |
Target | HTR3A / 5-HT3A Receptor |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, 0.05% Sodium Azide. PBS, 0.05% Sodium Azide |
Alternative names | HTR3A, 5-HT3-A, 5-HT3R-A, 5-HT3 receptor, 5HT3 ser Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B2042 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2042 was determined to be 5 ug/ml. |
Expiration Date | 12 months from date of receipt. |
ELISA, ICC, IF, IHC, IHC-P, WB | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |