HSV1 Antibody / Herpes Simplex Virus Type I

• Catalog Number: orb606484
• Category: Antibodies
• Description: The antibody reacts with HSV type 1 specific antigen. It is suitable for detection of HSV in human cellular material obtained from superficial lesions or biopsies and for the early identification of HSV in infected tissue cultures. The herpes simplex virus (HSV) (also known as cold sore, night fever or fever blister) is a virus that causes a contagious disease. There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The HSV-1 strain generally appears in the orafacial organs. HSV2 usually resides in the sacral ganglion at the base of the spine. All herpes viruses are morphologically identical: they have a large double-stranded DNA genome and the virion consists of an icosahedral nucleo-capsid, which is surrounded by a lipid bilayer envelope.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Virus
• Immunogen: Detergent-solubilized herpes simplex virus (HSV) type 1 infected cells were used as the immunogen for the recombinant HSV1 antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant HSV1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: HSV1/1934
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant HSV1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human cervix with recombinant HSV1 antibody (clone HSV1/1934). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant HSV1 antibody (clone HSV1/1934) as confirmation of integrity and purity.