HSPB8/H11/HSP22 Antibody

SKU: orb1538565

Description

HSPB8/H11/HSP22 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 5B12
• Immunogen: HSPB8 (NP_055180, 97 aa ~ 197 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: HSPB8 / H11 / HSP22
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.41 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPB8, Alpha-crystallin C chain, CMT2L, Crystallin alpha c, DHMN2, E2-induced gene 1 protein, CRYAC, E2IG1, HMN2, H11, Heat shock 27kDa protein 8, Heat shock protein beta-8, Heat shock 22kDa protein 8, HMN2A, HSP22, Protein kinase H11

Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody, clone 5B12.

Detection limit for recombinant GST tagged HSPB8 is approximately 3 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

HSPB8 / H11 / HSP22