HSPB8 Antibody

• Catalog Number: orb1258139
• Category: Antibodies
• Description: HSPB8 Antibody
• Target: HSPB8
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
• UniProt ID: Q9UJY1
• MW: Observed: 22kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2,
• Note: For research use only

Western blot analysis of extracts of various cell lines, using HSPB8 antibody (orb1258139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using HSPB8 antibody (orb1258139). Blue: DAPI for nuclear staining.