HSPB8 Antibody

SKU: orb1258139

Description

HSPB8 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
• Target: HSPB8
• Molecular Weight: Observed: 22kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27 kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, adult, autosomal dominant, CRYAC, PP1629

Western blot analysis of extracts of various cell lines, using HSPB8 antibody (orb1258139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using HSPB8 antibody (orb1258139). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

HSPB8

UniProt

Q9UJY1