HSPA5 Antibody

• Catalog Number: orb1271043
• Category: Antibodies
• Description: HSPA5 Antibody
• Target: HSPA5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
• UniProt ID: P11021
• MW: 72 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 78 kDa glucose-regulated protein, GRP-78, Endoplas
• Note: For research use only
• NCBI: P11021

Antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.

Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Confocal immunofluorescent analysis of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.