HSPA14 Antibody

SKU: orb1256591

Description

HSPA14 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Monkey, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2).
• Target: HSPA14
• Molecular Weight: Observed: 55kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock 70 kDa protein 14, HSP70-like protein 1, Heat shock protein HSP60, HSPA14, HSP60, HSP70L1

Western blot analysis of extracts of various cell lines, using HSPA14 antibody (orb1256591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of A549 cells using HSPA14 antibody (orb1256591). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

HSPA14

UniProt

Q0VDF9