Hsp90 Antibody

SKU: orb1788109

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: IHC: 1:200, WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,λ
• Immunogen: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 150-400 amino acids from human. Antigen Region: 150-400 aa.
• Target: HSP90AA1 (HGNC:5253)
• Molecular Weight: 84660 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA

Immunohistochemical analysis of paraffin-embedded Human Seminoma section using Pink1. Diluted at 1: 200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-Hsp90 Antibody at 1:1000 dilution. Lane 1: T47D whole cell lysates. Lane 2: A2058 whole cell lysates. Lane 3: HepG2 whole cell lysates. Lane 4: K562 whole cell lysates. Lane 5: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

HSP90AA1 (HGNC:5253)

UniProt

P07900