HSP90 Antibody (HRP)

SKU: orb151260

Description

Rabbit polyclonal to Hsp90 (HRP). Hsp90 is a highly conserved and essential stress protein Hsp90 is a highly conserved and essential stress protein...

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:500), IHC (1:100), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:500 dilution of SPC-104 was sufficient for detection of 0.2 mg of purified HSP90 by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Full length protein HSP90
• Target: HSP90
• Molecular Weight: 90kDa
• Purification: Protein A Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody. Load: 2 μgHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Quick Database Links

Gene Symbol

HSP90

UniProt

P08238

NCBI Gene ID

3326

RefSeq (Protein):NP_031381.2