HSP70/HSC70 Antibody (Biotin)

SKU: orb146786

Description

Mouse monoclonal to Hsp70 (Biotin). Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1:200), ICC/IF (1:200)
• Reactivity: Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Yeast
• Application Notes:
  1 µg/ml was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: BB70
• Immunogen: Chicken HSP70/HSP90 complex
• Target: HSP70/HSC70
• Molecular Weight: 73kDa
• Purification: Protein G Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPA1A, HSPA1B, HSPA1, HSPA8, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSC70, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein

Quick Database Links

Gene Symbol

HSP70/HSC70

UniProt

P08106

NCBI Gene ID

423504

RefSeq (Protein):NP_001006686.1