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HSP70 rabbit pAb Antibody
SKU: orb767029
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human HSP70 around the non-acetylation site of K246. |
| Molecular Weight | 70 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-HSPA1A antibody, anti-HSPA1 antibody, anti-HSPA1B antibody, anti-Heat shock 70 kDa protein 1A/1B antibody, anti-Heat shock 70 kDa protein 1/2 antibody, anti-HSP70-1/HSP70-2 antibody, anti-HSP70.1/HSP70.2 antibody
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Immunofluorescence analysis of human-kidney tissue. 1, HSP70 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-kidney tissue. 1, HSP70 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Western Blot analysis of MCF7 cells using HSP70 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HSP70 rabbit pAb Antibody (orb767029)
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