HSP70 Antibody

SKU: orb1822451

Description

Rabbit Anti-Human HSP70 Polyclonal

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Fish, Guinea pig, Hamster, Human, Mammal, Monkey, Mouse, Other, Parasite, Plant, Porcine, Rat, Sheep
• Application Notes:
  A 1:1000 dilution was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Full length Human HSP70 Protein
• Target: HSP70
• Molecular Weight: 70kDa
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Western blot analysis of Human, Rat brain cell lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody. Load: 2 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Western blot analysis of Mouse Pam212 cells showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Quick Database Links

Gene Symbol

HSP70

UniProt

P0DMV8, P0DMV9

NCBI Gene ID

3303

RefSeq (Protein):NP_005336.3