HSP70 Antibody (Acetyl Lys77): APC

• Catalog Number: orb414153
• Category: Antibodies
• Description: Rabbit polyclonal antibody against HSP70 Acetyl Lys77 conjugated to APC
• Target: HSP70 Acetyl Lys77
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Concentration: 0.5mg/mL
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• UniProt ID: P0DMV9, P0DMV8
• MW: 70 kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000); ELISA (1:1000); ICC/IF (1:60); IHC (1:50)
• Application notes: A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: HSP70 Acetylated lysine 77 antibody, HSP70 acLys77
• Research Area: Cancer Biology, Cell Biology, Protein Biochemistry
• Note: For research use only
• Entrez: 3303
• NCBI: NP_005336.3

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 μM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 Acetyl Lys77 protein using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.