HSP60 rabbit pAb

SKU: orb765459

Description

Rabbit polyclonal antibody to HSP60

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP60. AA range:511-560
• Molecular Weight: 68
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-HSPD1 antibody, anti-HSP60 antibody, anti-60 kDa heat shock protein antibody, anti- mitochondrial antibody, anti-60 kDa chaperonin antibody, anti-Chaperonin 60 antibody, anti-CPN60 antibody, anti-Heat shock protein 60 antibody, anti-HSP-60 antibody, anti-Hsp60 antibody, anti-HuCHA60 antibody, anti-Mitochondrial matrix protein P1 antibody, anti-P60 lymphocyte protein antibody

Immunofluorescence analysis of Hela cell. 1, HSP60 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of various cells using HSP60 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of COLO205 cells using HSP60 Polyclonal Antibody diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.