HSP60 Antibody (HRP)

SKU: orb151277

Description

Rabbit polyclonal to Hsp60 (HRP). In both prokaryotic and eukaryotic cells, the misfolding In both prokaryotic and eukaryotic cells, the misfolding...

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Bovine, Canine, Gallus, Hamster, Human, Mouse, Rabbit, Rat
• Application Notes:
  1 µg/ml of SPC-105 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human HSP60 produced through recombinant DNA methods in E.coli
• Target: HSP60
• Molecular Weight: 60kDa
• Purification: Protein A Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSPD1, HSP60, 60 kDa heat shock protein, mitochondrial, Chaperonin 60, CPN60, HuCHA60, Heat shock protein family D member 1, GroEL homolog, mitochondrial, GROEL, HLD4, HSP 60, HSP65, SPG 13

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody. Tissue: Adrenal. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody.

Western blot analysis of Human, Dog, Mouse SKBR3, MDCK, and MEF cell line lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody. Lane 1: Recom. Human Hsp60 (100ng), Lane2, 3 and 4: SKBR3 lysate (human), MDCK lysate (dog) and MEF lysate (mouse) (al at 7.5ug). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Quick Database Links

Gene Symbol

HSP60

UniProt

P10809

NCBI Gene ID

3329

RefSeq (Protein):NP_002147.2