HSP22 Antibody (Biotin)

SKU: orb151981

Description

Rabbit polyclonal to Hsp22 (Biotin). Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs. The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macro autophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L)..

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100), IHC (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of HSP22 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human HSP22
• Target: HSP22
• Molecular Weight: 22kDa
• Purification: Peptide Affinity Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPB8, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, E2-induced gene 1 protein, Protein kinase H11, Small stress protein-like protein, Heat shock protein family B member 8, CRYAC, E2IG1, CMT2L, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8

Quick Database Links

Gene Symbol

HSP22

UniProt

Q9UJY1

NCBI Gene ID

26353

RefSeq (Protein):NP_055180.1