HSP110 Antibody: APC

• Catalog Number: orb152133
• Category: Antibodies
• Description: Rabbit polyclonal to Hsp110 (APC). Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain. Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol. In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70. Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC
• Reactivity: Bovine, Fish, Hamster, Human, Monkey, Mouse, Rat, Sheep, Yeast
• Immunogen: Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100)
• Conjugation: APC
• MW: 110kDa
• Target: HSP110
• Entrez: 10808
• UniProt ID: Q92599
• NCBI: NP_006635.3
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: Heat shock 105kDa alpha antibody, heat shock 105kD
• Note: For research use only
• Application notes: 1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.

Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.