HSP β2 rabbit pAb

• Catalog Number: orb765454
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSP beta 2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSPB2. AA range:121-170
• MW: 24
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HSPB2 antibody, anti-Heat shock protein beta-
• Note: For research use only

Western Blot analysis of various cells using HSP β2 Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of A549 cells, using HSPB2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 and HeLa cells, using HSPB2 Antibody. The lane on the right is blocked with the synthesized peptide.