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HSF1 Antibody
SKU: orb1260257
Featured
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1). |
| Target | HSF1 |
| Molecular Weight | Observed: 90kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HSF1, HSTF1
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Western blot analysis of extracts of various cell lines, using HSF1 antibody (orb1260257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HSF1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HSF1 Antibody (orb1260257)
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