HSF1 Antibody

• Catalog Number: orb1260257
• Category: Antibodies
• Description: HSF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 90kDa
• Target: HSF1
• UniProt ID: Q00613
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HSF1, HSTF1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HSF1 antibody (orb1260257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.