hRIP3 Antibody

• Catalog Number: orb1239442
• Category: Antibodies
• Description: hRIP3 Antibody
• Target: Ripk3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/ml
• Buffer/Preservatives: hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Immunogen: Anti-hRIP3 antibody (orb1239442) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3. The immunogen is located within the last 50 amino acids of hRIP3.
• UniProt ID: Q9Y572
• MW: Predicted: 57kDObserved: 57 kD
• Tested applications: ELISA, IF, IHC-P, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP
• Note: For research use only
• NCBI: Q9Y572

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: hRIP3, orb1239442 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: hRIP3, orb1239442 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation of hRIP3 in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: hRIP3, orb1239442 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1-2: human THP-1 cell lysate in the absence (Lane 1) or the presence (Lane 2) of blocking peptide.

Immunofluorescence Validation of hRIP3 in Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling hRIP3 with orb1239442 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of hRIP3 in Human Breast Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast tissue labeling hRIP3 with orb1239442 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of hRIP3 in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon tissue labeling hRIP3 with orb1239442 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of hRIP3 in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-hRIP3 antibody (orb1239442) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of hRIP3 in Human Breast Tissue. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hRIP3 antibody (orb1239442) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of hRIP3 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (orb1239442) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.