HPX Antibody (Center)

SKU: orb1929911

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500, FC - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD30940
• Immunogen: This HPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the Central region of human HPX. Antigen Region: 200-227 aa.
• Target: HPX
• Molecular Weight: 51676 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hemopexin, Beta-1B-glycoprotein, HPX

HPX Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-HPX Antibody (Center) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-HPX Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Human plasma lysate. Lane 3: Mouse plasma lysate. Lane 4: Rat plasma lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

HPX

UniProt

P02790

RefSeq (Protein):NP_000604.1