HPV-16 E6 Antibody

• Catalog Number: orb750090
• Category: Antibodies
• Description: Reacts with E6 protein of human papilloma virus type 16 (HPV-16). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HPV16/1296
• Tested applications: IHC-P
• Reactivity: Virus
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human papilloma virus type 16 E6 protein was used as the immunogen for the HPV-16 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HPV-16 E6 antibody is available for research use only.
• UniProt ID: Not Known
• Storage: Store the HPV-16 E6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HPV-16 E6 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cervix with HPV-16 E6 antibody (clone HPV16/1296). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.