HP1BP3 Polyclonal Antibody

SKU: orb1418957

Description

Rabbit polyclonal antibody to HP1BP3.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heterochromatin protein 1-binding protein 3, Protein HP1-BP74, HP1BP3

Sample: Jurkat cell (human) Lysate at 30 ug. Panc1 cell (human) Lysate at 30 ug. Primary: Anti- HP1BP3 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 61kD. Observed band size: 61kD.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HP1BP3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

Q5SSJ5