HOXc8 Polyclonal Antibody

SKU: orb1421437

Description

Rabbit polyclonal antibody to HOXc8.

Research Area

Developmental Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Target: HOXC8
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein Hox-C8, Homeobox protein Hox-3A, HOXC8, HOX3A

Sample: Liver (Rat) Lysate at 40 ug. Primary: Anti-HOXc8 at 1/500 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 27 kD. Observed band size: 27 kD.

Sample: Brain (Rat) lysate at 30ug; Liver (Rat) lysate at 30ug; Primary: Anti-HOXc8 at 1: 200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 3000; Predicted band size: 27kD. Observed band size: 27kD, 43kD.

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30 min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

HOXC8

UniProt

P31273