HOXB2 Antibody

SKU: orb1410121

Description

Mouse monoclonal antibody to HOXB2 (Transcription Factor)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PCRP-HOXB2-1F2
• Immunogen: Recombinant fragment (aa114-220) of human HOXB2
• Target: HOXB2
• Source/Expression System: Bioreactor
• Molecular Weight: 37.9kDa
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

homeo box 2H; homeo box B2; homeobox B2; Homeobox protein Hox-2.8; Homeobox protein Hox-2H; Homeobox protein Hox-B2; Hox 2 8; Hox 2H; HOX2; HOX2H; hoxb2; HXB2_HUMAN; K8; K8 home protein

Analysis of Protein Array containing more than 19000 full-length human proteins using HOXB2-Monospecific Mouse Monoclonal Antibody (PCRP-HOXB2-1F2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 Mouse Monoclonal Antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using PCRP-HOXB2-1F2 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using PCRP-HOXB2-1F2 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Mouse Monoclonal Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Quick Database Links

Gene Symbol

HOXB2

UniProt

P14652