HOXA9 Antibody

• Catalog Number: orb1249108
• Category: Antibodies
• Description: HOXA9 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Human, Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: PDFSPCSFQSKA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 36kDa band observed in Mouse Spleen lysates (calculated MW of 29.9kDa according to NP_034586.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Quéré et al, Blood. 2011 Jun 2;117(22):5918-30. PMID: 21471525). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HOXA9
• UniProt ID: P31269
• NCBI: NP_689952.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HOXA9, homeobox A9, ABD-B, HOX1, HOX1.7, HOX1G, MG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 36kDa band observed in Mouse Spleen lysates (calculated MW of 29.9kDa according to NP_034586.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Quéré et al, Blood. 2011 Jun 2;117(22):5918-30. PMID: 21471525). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1249108 (2 ug/ml) staining of Moue Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.