HOXA10 Antibody

SKU: orb1248341

Description

HOXA10 Antibody

Research Area

Cancer Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37-38kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 42.4kDa according to Human NP_061824.3 and 43.2kDa according to Mouse NP_032289.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining preferentially in the spermatocytes and spermatids. Recommended concentration, 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: PQATSCSFAQNIKE
• Target: HOXA10
• Molecular Weight: Approx 38kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HOXA10, homeobox A10, HOX1, HOX1.8, HOX1H, PL, homeo box A10, homeobox protein 1H, homeobox protein HOXA10, homeobox protein Hox-1.8, homeobox protein Hox-1H, homeobox protein Hox-A10

orb1248341 (0.2 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248341 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248341 (0.3 ug/ml) staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

HOXA10

UniProt

P31260

RefSeq (Protein):NP_061824.3