Horse IgM mu chain Antibody Peroxidase Conjugated

SKU: orb346637

Description

Horse IgM mu chain antibody (Peroxidase)

Images & Validation

• Tested Applications: DOT, ELISA
• Dilution range: Anti-Horse IgM Peroxidase conjugate has been tested by ELISA and dot blot.
• Reactivity: Equine
• Application Notes:
  Anti-Horse IgM Peroxidase conjugate has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Horse IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Horse IgM mu heavy chain
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgM and Horse Serum. No reaction was observed against Horse IgG.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 10.0 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-Horse IgM mu chain Peroxidase Conjugated Antibody, rabbit anti-Horse IgM (mu chain) HRP Conjugated Antibody, rabbit anti-Horse IgM (mu chain) Antibody Peroxidase Conjugated, rabbit anti-Horse IgM mu chain Antibody HRP Conjugated

Dot Blot results of Rabbit Anti-Horse IgM Antibody Peroxidase Conjugated. Dots are Horse IgM at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit Anti-Horse IgM Antibody HRP at 10 µg/ml for 1hr at RT. Secondary Antibody: none.