Horse IgG F(ab')2 Antibody Peroxidase Conjugated

• Catalog Number: orb347163
• Category: Antibodies
• Description: Horse IgG F(ab')2 antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Equine
• Form/Appearance: Lyophilized
• Concentration: 1.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG F(c).
• Immunogen: Horse IgG F(ab')2 fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application notes: Anti-Horse IgG F(ab')2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit Anti-Horse IgG Fab2 Fragment peroxidase Con
• Note: For research use only

ELISA Results of Purified Rabbit Anti-Horse IgG F(ab')2 Antibody Peroxidase Conjugated tested against purified Horse IgG F(ab')2. Each well was coated in duplicate with 10 µg of Horse IgG F(ab')2 (p/n orb346178). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Horse IgG F(ab')2 HRP conjugated, and TMB substrate (p/n orb348651).