F(ab')2 Horse IgG F(ab')2 Antibody Peroxidase Conjugated

• Catalog Number: orb346774
• Category: Antibodies
• Description: F(ab')2 Horse IgG F(ab')2 antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: HRP
• Reactivity: Equine
• Form/Appearance: Lyophilized
• Concentration: 10.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
• Immunogen: Horse IgG F(ab')2 fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application notes: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit F(ab')2 anti-Horse IgG F(Ab')2 Antibody per
• Note: For research use only