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F(ab')2 Horse IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb348155

Description

F(ab')2 Horse IgG (H&L) antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
ReactivityEquine
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG F(ab')2
ImmunogenHorse IgG whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
ConjugationHRP

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration1.0 mg/mL
DisclaimerFor research use only

Alternative Names

Rabbit F(ab')2 Anti-Horse IgG Antibody Peroxidase Conjugation, Rabbit F(ab')2 Anti-Horse IgG Peroxidase Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG HRP Conjugated Antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

F(ab')2 Horse IgG (H&L) Antibody Peroxidase Conjugated (orb348155)

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500 μg
$ 490.00
DispatchUsually dispatched within 5-10 working days
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