F(ab')2 Horse IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb348155
• Category: Antibodies
• Description: F(ab')2 Horse IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: HRP
• Reactivity: Equine
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Immunogen: Horse IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit F(ab')2 Anti-Horse IgG Antibody Peroxidase
• Note: For research use only