Horse IgG F(ab')2 antibody

Catalog Number: orb347136

Catalog Number	orb347136
Category	Antibodies
Description	Horse IgG F(ab')2 antibody
Species/Host	Goat
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Equine
Isotype	IgG
Immunogen	Horse IgG F(ab')2 fragment
Concentration	2.1 mg/mL
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/Appearance	Liquid (sterile filtered)
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG F(c).
Conjugation	Unconjugated
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Alternative names	Goat Anti Horse IgG Fab2 Fragment Antibody, Goat A Read more...
Note	For research use only
Application notes	Anti-Horse IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Expiration Date	12 months from date of receipt.

Applications:	IP
Conjugation:	Agarose
Purity:	This product is a purified Horse IgG isolated from normal Horse Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Horse IgG.
Source:	Horse

Sizes Available: 5 mg

Applications:	SDS-PAGE
Conjugation:	Unconjugated
Purity:	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
Source:	Horse

Sizes Available: 2 mg

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
Source:	Horse

Sizes Available: 1 mg

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
Source:	Horse

Sizes Available: 1 mg

Conjugation:	Biotin
Purity:	This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
Source:	Horse

Sizes Available: 1 mg