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Catalog Number | orb346178 |
---|---|
Category | Proteins |
Description | Horse IgG F(ab')2 Antibody |
Tested applications | SDS-PAGE |
Isotype | IgG F(ab')2 |
Concentration | 2.0 mg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin. |
Conjugation | Unconjugated |
Source | Horse |
Biological Origin | Horse |
Storage | Store vial at 4° C upon receipt. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Horse IgG F(ab')2 Fragment, Horse IgG Fab2 Fragmen Read more... |
Note | For research use only |
Application notes | Horse IgG F(ab')2 has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS PAGE Results of Horse F(ab')2 Fragment. Lane 1: 1x Reduced, Horse F(ab')2 Fragment. Lane 2: Molecular Weight Ladder. 4-20% SDS-PAGE; Coomassie Stained.
ELISA, IHC, WB | |
Equine | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Equine | |
Rabbit | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Equine | |
Goat | |
Polyclonal | |
HRP |
IP | |
Agarose | |
This product is a purified Horse IgG isolated from normal Horse Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Horse IgG. | |
Horse |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin. | |
Horse |