HNRNPA1 Antibody

SKU: orb1256354

Description

HNRNPA1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).
• Target: HNRNPA1
• Molecular Weight: Observed: 39kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

HNRPA1, ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, hnRNP A1, hnRNP-A1

Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (orb1256354) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunohistochemistry of paraffin-embedded rat spinal cord using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human brain astrocytoma using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using HNRNPA1 antibody (orb1256354).

Quick Database Links

Gene Symbol

HNRNPA1

UniProt

P09651