HNRNPA1 Antibody

• Catalog Number: orb1256354
• Category: Antibodies
• Description: HNRNPA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 39kDa
• Target: HNRNPA1
• UniProt ID: P09651
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HNRPA1, ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, h
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (orb1256354) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunohistochemistry of paraffin-embedded rat spinal cord using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human brain astrocytoma using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using HNRNPA1 antibody (orb1256354).