HNMT Antibody (N-term)

• Catalog Number: orb1935816
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P50135
• MW: 33295 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB12380
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histamine N-methyltransferase, HMT, HNMT
• Research Area: Neuroscience
• Note: For research use only
• NCBI: NP_001019246.1, NP_008826.1, NP_001019245.1

Western blot analysis of HNMT Antibody (N-term) in Hela cell line lysates (35 ug/lane). HNMT (arrow) was detected using the purified polyclonal antibody.

Confocal immunofluorescent analysis of HNMT Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.