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Description
Research Area
Images & Validation
−| Tested Applications | ICC, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:10000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary. |
| Target | HNF1 alpha |
| Molecular Weight | 67 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Buffer/Preservatives | PBS, 20% Glycerol, 0.025% ProClin 300. |
| Concentration | 0.65 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunofluorescence analysis of HepG2 cells using HNF1 alpha antibody

Immunohistochemical staining of A549 xenograft using HNF1 alpha antibody

Immunoprecipitation analysis of HepG2 whole cell using HNF1 alpha antibody

Western blot analysis of Rat tissue using HNF1 alpha antibody

Western blot analysis of Mouse tissue using HNF1 alpha antibody
Quick Database Links
UniProt Details
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Protocol Information
HNF1 alpha Rabbit Polyclonal Antibody (orb556884)
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