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Catalog Number | orb1935482 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This HN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human HN1. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | Q9UK76 |
MW | 16015 Da |
Tested applications | FC, IF, WB |
Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB32984 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Hematological and neurological expressed 1 protein Read more... |
Note | For research use only |
NCBI | NP_057269.1, NP_001002032.1, NP_001002033.1 |
HN1 Antibody (Center) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the HN1 antibody detected the HN1 protein (arrow).
HN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of HN1 Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).