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HN1 Antibody (Center)

SKU: orb1935482

Description

HN1 Antibody (Center)

Research Area

Cell Biology

Images & Validation

Tested ApplicationsFC, IF, WB
Dilution RangeWB - 1:1000, IF - 1:10-50, FC - 1:10-50
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
Molecular Weight16015 Da
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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HN1 Antibody (Center)

HN1 Antibody (Center) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the HN1 antibody detected the HN1 protein (arrow).

HN1 Antibody (Center)

HN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HN1 Antibody (Center)

Confocal immunofluorescent analysis of HN1 Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

HN1 Antibody (Center) (orb1935482)

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Available Sizes

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50 μl
$ 170.00
100 μl
$ 310.00
DispatchUsually dispatched within 5-10 working days
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