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HMMR Antibody (C-term)
Description
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 84100 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
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All lanes: Anti-HMMR Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysates. Lane 2: LNCaP whole cell lysates. Lane 3: HL-60 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

HMMR Antibody (C-term) western blot analysis in Ramos cell line lysates (35 ug/lane). This demonstrates the HMMR antibody detected the HMMR protein (arrow).

HMMR Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HMMR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HMMR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Protocol Information
HMMR Antibody (C-term) (orb1937966)
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