HMMR Antibody (C-term)

• Catalog Number: orb1937966
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This HMMR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-697 amino acids from the C-terminal region of human HMMR.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: O75330
• MW: 84100 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Clone Number: RB17647
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hyaluronan mediated motility receptor, Intracellul
• Note: For research use only
• NCBI: NP_036616.2, NP_001136028.1, NP_001136029.1, NP_036617.2

All lanes: Anti-HMMR Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysates. Lane 2: LNCaP whole cell lysates. Lane 3: HL-60 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

HMMR Antibody (C-term) western blot analysis in Ramos cell line lysates (35 ug/lane). This demonstrates the HMMR antibody detected the HMMR protein (arrow).

HMMR Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HMMR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HMMR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.