HMGCS1 Antibody (C-term)

• Catalog Number: orb36825
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Target: HMGCS1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Hamster, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: Q01581
• MW: 57294
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB20147
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Hydroxymethylglutaryl-CoA synthase antibody,
• Research Area: Cancer Biology, Cardiovascular Research, Metabolis
• Note: For research use only
• NCBI: NP_001091742.1, NP_002121.4

References

Jonckheere, Veronique et al. Omics Assisted N-terminal Proteoform and Protein Expression Profiling On Methionine Aminopeptidase 1 (MetAP1) Deletion Mol Cell Proteomics, 17, 694-708 (2018)

PubMed: 29317475

Western blot analysis of HMGCS1 antibody (C-term) in K562 cell line lysates (35 ug/lane). NKRF (arrow) was detected using the purified Pab.

Flow cytometric analysis of hela cells using HMGCS1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.