HMGB2 Rabbit mAb [KD Validated] Antibody

SKU: orb1951002

Description

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human HMGB2
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HMG 2; HMG B2; HMG-2; HMG2; HMGB2;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.

Quick Database Links

UniProt

P26583