HMGB1 Rabbit Polyclonal Antibody

SKU: orb627736

Description

Rabbit polyclonal antibody to HMGB1

Research Area

Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: high-mobility group box 1
• Target: HMGB1
• Molecular Weight: 26 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Jurkat cells were subjected to SDS PAGE followed by western blot with orb627736 (HMGB1 antibody) at dilution of 1:1000.

Immunofluorescence analysis of HeLa cells using orb627736 (HMGB1 antibody) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human breast cancer using orb627736 (HMGB1 antibody) at dilution of 1:100.

Quick Database Links

Gene Symbol

HMGB1

UniProt

P09429