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Item 1 of 6
Item 1 of 6
HMGB1 Antibody, KO Validated
Catalog Number: orb1274463
| Catalog Number | orb1274463 |
|---|---|
| Category | Antibodies |
| Description | HMGB1 Antibody, KO Validated |
| Target | HMGB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1). |
| UniProt ID | P09429 |
| MW | Observed: 29kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobi Read more... |
| Note | For research use only |
Western blot analysis of extracts of various cell lines, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGB1 antibody (orb1274463) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
