HLA-DRB1 Antibody (N-term)

• Catalog Number: orb1929737
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21019
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Target: This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids of human HLA-D_1.
• UniProt ID: P04229
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HLA class II histocompatibility antigen, DRB1-1 be
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of HLA-DRB1 Antibody (N-term) in WiDr cell line lysates (35 ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab.

Western blot analysis of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Flow cytometric analysis of widr cells using HLA-DRB1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.