HLA-DR (MHC II) Antibody

• Catalog Number: orb1410572
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-DR
• Target: HLA-DRA
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG3
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Immunogen: Activated human peripheral blood mononuclear cells
• UniProt ID: P01903
• MW: ~28kDa (beta chain)
• Tested applications: FC, IF, IHC, WB
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (0.5-1ug/ml); Western Blot (1-2ug/ml);,Immunohistochemistry (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Ramos, Daudi or HuT78 cells. Human tonsil or lymph node.
• Clone Number: SPM289
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DRB1; DRB4; HLA class II histocompatibility antige
• Note: For research use only
• Entrez: 3122
• Expiration Date: 12 months from date of receipt.