HLA-DR Antibody (MHC II)

• Catalog Number: orb606901
• Category: Antibodies
• Description: Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain. Polymorphisms yield hundreds of HLA-B and -C alleles.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human Bristol 8 cells were used as the immunogen for the HLA-DR antibody.
• UniProt ID: P01903
• Tested applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the HLA-DR antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: TAL 1B5
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This HLA-DR antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of human Raji cells with HLA-DR antibody (clone TAL 1B5); Red = isotype control, Blue = HLA-DR antibody.

IHC staining of FFPE human prostate cancer with HLA-DR antibody (clone TAL 1B5). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer with HLA-DR antibody (clone TAL 1B5). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HLA-DR antibody (clone TAL 1B5) as confirmation of integrity and purity.