HLA-DQA2 Antibody

• Catalog Number: orb1249032
• Category: Antibodies
• Description: HLA-DQA2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QSHGPSGQYTH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in Human Bone Marrow lysates (calculated MW of 28.0kDa according to NP_064440.1). Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HLA-DQA2
• UniProt ID: P01906
• NCBI: NP_064440.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HLA-DQA2, major histocompatibility complex, class
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in Human Bone Marrow lysates (calculated MW of 28.0kDa according to NP_064440.1). Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249032 (0.3 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249032 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.