HLA-DQA1 Antibody (N-term)

• Catalog Number: orb1939545
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32210
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 27805 Da
• Target: This HLA-DQA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human HLA-DQA1.
• UniProt ID: P01909
• NCBI: NP_002113.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HLA class II histocompatibility antigen, DQ alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-HLA-DQA1 Antibody (N-term) at 1:1000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

HLA-DQA1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HLA-DQA1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the HLA-DQA1 antibody detected the HLA-DQA1 protein (arrow).

Western blot analysis of HLA-DQA1 (arrow) using rabbit polyclonal HLA-DQA1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DQA1 gene.