HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43]

• Catalog Number: orb1252224
• Category: Antibodies
• Description: HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43]
• Target: HLA-DPB1, HLA-DQA1
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
• UniProt ID: P01903, P01908, P01909, P04440, P01920
• Tested applications: IF, IHC-P, WB
• Application notes: WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CR3/43
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1 ug/10^6 cells (blocked with goat sera); Red = isotype control, Blue = HLA-DP/DQ/DR antibody.