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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone No. | CR3/43 |
| Immunogen | Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody. |
| Target | HLA-DPB1, HLA-DQA1 |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Concentration | 0.2 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).
SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.
IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1 ug/10^6 cells (blocked with goat sera); Red = isotype control, Blue = HLA-DP/DQ/DR antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
HLA-DPB1, HLA-DQA1 Antibody (orb1252224)
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