HLA-A, HLA-B, HLA-C Antibody

SKU: orb2568759

Description

The HLA-A,B,C mAb (W6/32) monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. MHC class I plays a crucial role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide MHC class I, which induces CD8 T cell activation and subsequent cell killing. CD8 molecules also bind to MHC class I, which enhances TCR signaling. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer cells (NK cells) and promotes self-tolerance.

Images & Validation

• Reactivity: Human
• Application Notes:
  Recomended Isotype: Mouse IgG2A k Isotype Control, Recomended Isotype Catalog: orb2568793

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: m IgG2a k
• Clone No.: W6/32
• Immunogen: Animals were immunized with cells from human tonsils. Spleen cells were fused with P3X63Ag8 myeloma cells
• Target: HLA-A, HLA-B, HLA-C
• Purity: ≥ 95 % (CGE, reducing conditions) ≤ 5 % aggregates (analytical SEC) Endotoxin level ≤ 1 EU/mg
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 1 mg/ mL
• Disclaimer: For research use only

Alternative Names

HLA ABC, anti-HLA Class 1, anti HLA I, Anti-HLA class I

Quick Database Links

Gene Symbol

HLA-A, HLA-B, HLA-C

UniProt

015506