HLA-ABC Antibody (MHC I)

SKU: orb2637312

Description

Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: FACS, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HLA-ABC antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: SPM419
• Immunogen: Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free HLA-ABC antibody (clone SPM419) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01889, P30499, P30443